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Bio-Rad
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Image Search Results
Journal: Development (Cambridge, England)
Article Title: Synchronous and symmetric migration of Drosophila caudal visceral mesoderm cells requires dual input by two FGF ligands.
doi: 10.1242/dev.068791
Figure Lengend Snippet: Fig. 3. FGF signaling supports CVM cell survival. Embryos are oriented with anterior towards the left and dorsal upwards. (A-C)Embryos containing the croc-lacZ reporter gene stained using anti-bgal (red) and anti-FasIII (green) to identify CVM and TVM cells, respectively. Wild-type (stage 13; A), Df(2R)BSC25 (stage 13; B) and Df(2R)BSC25 embryos expressing p35 via G447.Gal4 driver (stage 13; C) are depicted. (C)Expression of the anti-apoptotic protein p35 rescues the morphology of CVM cells and allows cells to migrate anteriorly, but cells (asterisk) fail to reach the anterior-most position of the TVM (white line). (D-F)Cell death is observed in FGF mutants using the TUNEL assay. Wild-type embryo (i.e. G447.Gal4 UAS-CD2) of stage 13 (D) and Df(2R)BSC25 G447.Gal4 UAS-CD2 embryos of stage 12 (E) and stage 13 (F) stained with anti-CD2 to detect CVM cells (red) and apoptosis using the TUNEL assay (green).
Article Snippet: The following antibodies were used in the study: rabbit anti-b-gal antibody (1:400; Molecular Probes), mouse anti-Fas III antibody (1:10; Developmental Studies Hybridoma Bank),
Techniques: Staining, Expressing, TUNEL Assay
Journal:
Article Title: Effect of an inhibitor of Jun N-terminal protein kinase, SP600125, in single allergen challenge in sensitized rats
doi: 10.1111/j.1365-2567.2004.01887.x
Figure Lengend Snippet: Effect of SP600125 on eosinophil and T-lymphocyte subset (CD2+, CD4+ and CD8+) counts in airway submucosa per mm of basement membrane for groups as detailed in Fig. 1. Allergen challenge caused a significant increase in T lymphocytes expressing CD2+. (*P < 0·05 as compared to saline group), and in MBP + eosinophils (***P < 0·001 as compared to the saline group). Data are shown as mean ± SEM.
Article Snippet: For staining of CD2 + ,
Techniques: Membrane, Expressing, Saline